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A Spectrofluorimetric Assay of Calmodulin-Dependent Protein Phosphatase Using 4-methylumbelliferyl Phosphate

F A Anthony, D L Merat, W Y Cheung

Anal Biochem. 1986 May 15;155(1):103-7.

PMID: 3717545

Abstract:

A continuous spectrofluorimetric assay of calmodulin-dependent phosphatase is described. The assay monitors the formation of a fluorescent 4-methylumbelliferone from the dephosphorylation of 4-methylumbelliferyl phosphate and detects as little as 1 pmol of 4-methylumbelliferone. The phosphatase shows a Km of 1.3 mM for the substrate and a Vmax of 100 nmol/mg/min.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP3368045-A 4-Methylumbelliferyl phosphate 4-Methylumbelliferyl phosphate 3368-04-5 Price
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