0

A TGF-β Receptor 1 Inhibitor for Prevention of Proliferative Vitreoretinopathy

Khaled Nassar, Swaantje Grisanti, Aysegul Tura, Julia Lüke, Matthias Lüke, Mahmoud Soliman, Salvatore Grisanti

Exp Eye Res. 2014 Jun;123:72-86.

PMID: 24742493

Abstract:

This study evaluates the use of the TGF-β receptor 1 inhibitor LY-364947 (LY) to prevent proliferative vitreoretinopathy (PVR). For the in vitro experiments Human Tenon's Fibroblasts (HTFs) and retinal pigment epithelial (RPE) cells were treated with different concentrations of LY to determine HTF proliferation and RPE transdifferentiation. For in vivo testing 30 rabbits underwent a PVR trauma model. The animals received different concentrations of intravitreally injected LY, with or without vitrectomy. LY treatment reduced HTF proliferation and RPE transdifferentiation in vitro. In vivo intravitreal injection of LY prevented PVR development significantly. This positive effect was also present when LY injection was combined with vitrectomy. Intravitreal injection of LY prevented tractional retinal detachment in 14 out of 15 animals. In conclusion, treatment with the TGF-β receptor 1 inhibitor LY reduces HTF proliferation and RPE transdifferentiation in vitro and prevents proliferative vitreoretinopathy and subsequent tractional retinal detachment in vivo.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP396129536 LY-364947 LY-364947 396129-53-6 Price
qrcode