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A Thermostable Pyrimidine Nucleoside Phosphorylase From Brevibacillus Borstelensis LK01 for Synthesizing Halogenated Nucleosides

Ke Liu, Youzhi Zhou, Jinsong Zhang, Jianlin Chu, Yewei Zhang, Bingfang He

Biotechnol Lett. 2017 Dec;39(12):1903-1910.

PMID: 28871515

Abstract:

Objective:
To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.
Results:
BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2'-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61-84%.
Conclusion:
An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9055350 Pyrimidine nucleoside phosphorylase Pyrimidine nucleoside phosphorylase 9055-35-0 Price
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