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A Tryptophan C-methyltransferase Involved in Streptonigrin Biosynthesis in Streptomyces Flocculus

D L Hartley, M K Speedie

Biochem J. 1984 May 15;220(1):309-13.

PMID: 6743267

Abstract:

A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP3930196 Streptonigrin from Streptomyces flocculus Streptonigrin from Streptomyces flocculus 3930-19-6 Price
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