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ABO-blood-group-related Idiotypic Network: Mimicry of Oligosaccharide Epitope by Rabbit Antiidiotypic Antibodies to Murine Monoclonal anti-A Antibody

D Rochu, H Crespeau, A Fine, P Gane, P Rouger, C Salmon, J M Fine

Res Immunol. May-Jun 1990;141(4-5):373-87.

PMID: 1702553

Abstract:

The idiotypy of antibodies (Ab) specific for oligosaccharide determinants of blood groups of the human ABO system was studied through a cascade. Xenogenic antiidiotypic Ab (Ab2) raised in rabbits to the murine monoclonal anti-A61 (Ab1) were screened for reactivity with various anti-ABH Ab. Three anti-A and three anti-A,B monoclonal antibodies (mAb) which were developed in the same mouse strain as that producing Ab1, as well as a human polyclonal anti-A, were found to share cross-reactive idiotopes (CRI) with Ab1. CRI on murine mAb could be due to a Biozzi recurrent Id on anti-A Ab reacting with anti-Id "à la Oudin", while CRI on human anti-A Ab suggested the presence of paratope-induced anti-Id. Inhibition by Ab2 of haemagglutination of A, B or O human red blood cells by many murine anti-ABH mAb, and by polyclonal or monoclonal human anti-A, strongly supported the occurrence of anti-Id mimicking ABH epitopes belonging to type 2 determinants carried by human erythrocytes. Furthermore, a rabbit immunized with Ab2 produced a potent Ab3 response characterized by anti-H-type-2 specificity. Altogether, these results are consistent with the first successful production of anti-Id Ab that mimics the tridimensional shape of a well defined and strictly carbohydrate epitope, eliciting a haemagglutinating Ab3.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412978 Anti-CIDE-A (ab1) antibody produced in rabbit Anti-CIDE-A (ab1) antibody produced in rabbit Price
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