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Activation of O(2) Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated From Cotyledons of Castor Bean by Cis, trans-Abscisic Acid

Activation of O(2) Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated From Cotyledons of Castor Bean by Cis, trans-Abscisic Acid

T Tezuka, Y Yamamoto, N Kondo

Plant Physiol. 1990 Jan;92(1):147-50.

PMID: 16667237

Abstract:

The rate of O(2) uptake and the activity of NAD-specific isocitrate dehydrogenase (NAD-ICDH) of mitochondria isolated from castor bean cotyledons were increased by added cis, trans-abscisic acid (ABA) in an in vitro system, while the NADP-specific isocitrate dehydrogenase (NADP-ICDH) was not affected by cis, trans-ABA. Trans, trans-ABA showed only a slightly inhibitory effect on O(2) uptake. The V(max) value for the isotherm of isocitrate by the enzyme was also increased by cis, trans-ABA. The isocitrate K(m) value for the enzyme with cis, trans-ABA was calculated to be approximately 249.8 micromolar, while the S(0.5) for the enzyme without the ABA was 151.6 micromolar. The n value calculated from the slopes of Hill plots of the reaction velocity of NAD-ICDH against isocitrate concentration was 1.5 in the mitochondrial fraction in the absence of ABA, and cis, trans-ABA treatment decreased the value to 1.0. Cis, trans-ABA also partly overcame the inhibition of NAD-ICDH activity by ATP.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14398539	(−)-cis,trans-Abscisic acid		14398-53-9	Price

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