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Activators of G Proteins Inhibit GSK-3beta and Stabilize beta-Catenin in Xenopus Oocytes

Activators of G Proteins Inhibit GSK-3beta and Stabilize beta-Catenin in Xenopus Oocytes

S Mahmoud A Najafi

Biochem Biophys Res Commun. 2009 May 1;382(2):365-9.

PMID: 19285033

Abstract:

Frizzled proteins, the receptors for Wnt ligands have seven hydrophobic transmembrane domains, a structural feature of G protein coupled receptors. Therefore a role for G proteins in the regulation of Wnt signaling has been proposed. Here I have used Xenopus oocytes to study the role of heterotrimeric G proteins in the regulation of GSK-3beta and beta-Catenin, two essential components of the canonical Wnt pathway. In these cells, general activators of G proteins such as GTPgamma-S and AlF4(-) increase beta-Catenin stability and decrease GSK-3beta mediated phosphorylation of the microtubule associated protein, Tau. Among several members of Galpha proteins tested, expression of a constitutively active mutant of Galphaq (GalphaqQL) led to a significant increase in accumulation of beta-Catenin. The stabilization of beta-Catenin mediated by Galphaq was reversed by a Galphaq specific inhibitor, Gp-antagonist 2A, but not by a specific blocking peptide for Galphas. Expression of GalphaqQL also inhibited GSK-3beta-mediated tau phosphorylation in Xenopus oocytes. These results support a role for the Gq class of G proteins in the regulation of Wnt/beta-Catenin signal transduction.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248364	GP Antagonist-2A			Price

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