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Adenine Base Editors Catalyze Cytosine Conversions in Human Cells

Heon Seok Kim, You Kyeong Jeong, Junho K Hur, Jin-Soo Kim, Sangsu Bae

Nat Biotechnol. 2019 Oct;37(10):1145-1148.

PMID: 31548727

Abstract:

Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP71307-A Cytosine Cytosine 71-30-7 Price
AP73245-A Adenine Adenine 73-24-5 Price
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