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Adsorption of Polysorbate 20 and Proteins on Hydrophobic Polystyrene Surfaces Studied by Neutron Reflectometry

Adsorption of Polysorbate 20 and Proteins on Hydrophobic Polystyrene Surfaces Studied by Neutron Reflectometry

Zhenhuan Zhang, Sara Orski, Ann Marie Woys, Guangcui Yuan, Isidro E Zarraga, Norman J Wagner, Yun Liu

Colloids Surf B Biointerfaces. 2018 Aug 1;168:94-102.

PMID: 29724643

Abstract:

Understanding the adsorption of protein and surfactant molecules on hydrophobic surfaces is very important for storage stability and delivery of pharmaceutical liquid formulations as many commonly-used devices, such as drug containers and syringes, have hydrophobic surfaces. Neutron reflectometry is used here to investigate the structure information of the adsorption process of non-ionic surfactant (polysorbate 20) and proteins (monoclonal antibody (mAb) and lysozyme) on polystyrene surfaces. Thickness of adsorbed polysorbate 20 thin film is observed to be ≈21 Å, comparable to the radius of gyration of polysorbate 20 micelles in solution. Although no lysozyme adsorption is observed on the polystyrene surface in low solution pH condition, the mAb can be strongly absorbed on the polystyrene surface with a layer thickness of ≈145 Å. The mAb concentration near the surface is about 135 mg/ml significantly larger than the bulk protein concentration. The differences in adsorption behavior are attributed to different protein interactions with a hydrophobic surface. Further, both surfactants and proteins adsorbed on the polystyrene surfaces can not be rinsed off using pure water.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9005645-A	Polysorbate 20		9005-64-5	Price

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