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Affinity Chromatography for Purification of Two Urokinases From Human Urine

Affinity Chromatography for Purification of Two Urokinases From Human Urine

R Takahashi, K Akiba, M Koike, T Noguchi, Y Ezure

J Chromatogr B Biomed Sci Appl. 2000 May 26;742(1):71-8.

PMID: 10892585

Abstract:

A new affinity chromatography (hydrophobic-mediated affinity chromatography), which was characterized by the matrix having both affinity site to urokinase and hydrophobic site, was established for the purification of urokinase from human urine. The hydrophobic affinity matrix (tentatively named PAS in the text) was prepared by immobilizing 6-aminocaproic acid on Sepharose CL-6B, followed by a coupling p-aminobenzamidine to a part of the hydrophobic site on the matrix. The PAS matrix was applied to the purification of urokinase from human urine, and high- and low-molecular weight pure urokinases were efficiently obtained in high yield by the present method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9039536	Urokinase from human urine		9039-53-6	Price

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