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Affinity Chromatography of Estrogen Receptors on Diethylstilbestrol-Agarose

T R Van Oosbree, U H Kim, G C Mueller

Anal Biochem. 1984 Feb;136(2):321-7.

PMID: 6721137

Abstract:

Diethylstilbestrol was coupled to epoxy-activated agarose yielding an affinity resin which is highly efficient for the isolation of estrogen receptors. This resin, diethylstilbestrol-agarose (DES-agarose), bound two proteins (Mr = 50,000 and 65,000) from rabbit uterine cytosol that show a specific interaction with estradiol. A two step procedure--adsorption on DES-agarose followed by a selective elution with p-sec-amylphenol and NaSCN, yielded highly purified estrogen receptors which can be used in the studies of estradiol-receptor interactions with other cell constituents.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410941 Epoxy-activated-Agarose Epoxy-activated-Agarose Price
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