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Affinity Elution From a Phosphonic acid-Sepharose Derivative in the Purification of Human Liver Alkaline Phosphatase

L E Seargeant, R A Stinson

J Chromatogr. 1979 May 11;173(1):101-8.

PMID: 546866

Abstract:

The compound p-aminobenzylphosphonic acid has been coupled via an azo linkage to tyraminyl-Sepharose 4B. This derivative at pH 6.0 bound most of the protein and all of the alkaline phosphatase in a crude preparation from human liver. The phosphatase was selectively eluted with the substrate 2-naphthylphosphate and a purification of 400-fold obtained. This step, when incorporated into a procedure for the purification of human liver alkaline phosphatase, yielded essentially pure enzyme.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP5424271 p-Aminobenzylphosphonic acid p-Aminobenzylphosphonic acid 5424-27-1 Price
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