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Affinity Purification Lipase From Wheat Germ: Comparison of Hydrophobic and Metal Chelation Effect

Affinity Purification Lipase From Wheat Germ: Comparison of Hydrophobic and Metal Chelation Effect

Kazım Köse, Kadir Erol, Dursun Ali Köse, Emre Evcı, Lokman Uzun

Artif Cells Nanomed Biotechnol. 2017 May;45(3):574-583.

PMID: 27033457

Abstract:

Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4 mg/g and 196.4 mg/g, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001621-I	Lipase from wheat germ		9001-62-1	Price

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