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Affinity Purification of Human Tau Proteins and the Construction of a Sensitive Sandwich Enzyme-Linked Immunosorbent Assay for Human Tau Detection

M Mercken, M Vandermeeren, U Lübke, J Six, J Boons, E Vanmechelen, A Van de Voorde, J Gheuens

J Neurochem. 1992 Feb;58(2):548-53.

PMID: 1729400

Abstract:

Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4248049 Anti-τ (Tau) antibody produced in rabbit Anti-τ (Tau) antibody produced in rabbit Price
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