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Aggregation Behavior of Fullerenes in Aqueous Solutions: A Capillary Electrophoresis and Asymmetric Flow Field-Flow Fractionation Study

Aggregation Behavior of Fullerenes in Aqueous Solutions: A Capillary Electrophoresis and Asymmetric Flow Field-Flow Fractionation Study

Alina Astefanei, Oscar Núñez, Maria Teresa Galceran, Wim Th Kok, Peter J Schoenmakers

Anal Bioanal Chem. 2015 Oct;407(26):8035-45.

PMID: 26314484

Abstract:

In this work, the electrophoretic behavior of hydrophobic fullerenes [buckminsterfullerene (C60), C70, and N-methyl-fulleropyrrolidine (C60-pyrr)] and water-soluble fullerenes [fullerol (C60(OH)24); polyhydroxy small gap fullerene, hydrated (C120(OH)30); C60 pyrrolidine tris acid (C60-pyrr tris acid); and (1,2-methanofullerene C60)-61-carboxylic acid (C60CHCOOH)] in micellar electrokinetic capillary chromatography (MECC) was evaluated. The aggregation behavior of the water-soluble compounds in MECC at different buffer and sodium dodecyl sulfate (SDS) concentrations and pH values of the background electrolyte (BGE) was studied by monitoring the changes observed in the electrophoretic pattern of the peaks. Broad and distorted peaks that can be attributed to fullerene aggregation were obtained in MECC which became narrower and more symmetric by working at low buffer and SDS concentrations (below the critical micelle concentration, capillary zone electrophoresis (CZE) conditions). For the characterization of the suspected aggregates formed (size and shape), asymmetrical flow field-flow fractionation (AF4) and transmission electron microscopy (TEM) were used. The results showed that the increase in the buffer concentration promoted the aggregation of the particles, while the presence of SDS micelles revealed multiple peaks corresponding to particles of different aggregation degrees. Furthermore, MECC has been applied for the first time for the analysis of C60 in two different cosmetic products (i.e., anti-aging serum and facial mask).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP155116191	(1,2-Methanofullerene C60)-61-carboxylic acid		155116-19-1	Price
LS76133	C60 Pyrrolidine tris-acid			Price

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