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Alanine Is a Precursor in the Biosynthesis of Fumonisin B1 by Fusarium Moniliforme

Alanine Is a Precursor in the Biosynthesis of Fumonisin B1 by Fusarium Moniliforme

B E Branham, R D Plattner

Mycopathologia. 1993 Nov;124(2):99-104.

PMID: 8008046

Abstract:

The biosynthesis of fumonisin B1 by Fusarium moniliforme was studied in liquid culture. Stable isotope labeled alanine was added as a precursor to static and shaken cultures of F. moniliforme. Incorporation of 13C and 2H labeled L-alanine into fumonisin B1 was measured by GC-MS. Under static culture conditions, 20.8 micrograms ml-1 of FB1 were produced with a 5.5% level of incorporation of intact, labeled alanine into fumonisin B1. Under shake culture conditions, much higher levels of fumonisin B1 were produced with levels reaching 159-240 micrograms ml-1 by 21 days after culture initiation. A lower level of alanine incorporation, from 1.1-1.4%, was observed under these conditions. Under shake conditions, incorporation of labeled alanine was reduced because of the rapid metabolism of these cultures combined with the high level of fumonisin B1 production resulted in rapid turnover of the added, labeled alanine and reduced percentage of incorporation. The evidence presented indicates that alanine is incorporated intact into fumonisin B1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP116355830-A	Fumonisin B1 from Fusarium moniliforme		116355-83-0	Price

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