Home
Resources
Publications
Alisol A 24-acetate Ameliorates Nonalcoholic Steatohepatitis by Inhibiting Oxidative Stress and Stimulating Autophagy Through the AMPK/mTOR Pathway

Alisol A 24-acetate Ameliorates Nonalcoholic Steatohepatitis by Inhibiting Oxidative Stress and Stimulating Autophagy Through the AMPK/mTOR Pathway

Chenqu Wu, Menghui Jing, Lijuan Yang, Lei Jin, Yicun Ding, Juan Lu, Qin Cao, Yuanye Jiang

Chem Biol Interact. 2018 Aug 1;291:111-119.

PMID: 29883724

Abstract:

Alisol A 24-acetate (AA), a natural triterpenoid isolated from the traditional Chinese medicine Rhizoma Alismatis, has various therapeutic effects. We investigated the anti-nonalcoholic steatohepatitis (NASH) effect of AA and its underlying mechanisms in vitro and in vivo. C57BL/6 mice were fed a methionine and choline-deficient (MCD) diet for 4 weeks to induce NASH. The mice were simultaneously treated with a daily dose of AA (15, 30, and 60 mg kg-1, ig) for 4 weeks. On the last day, the animals were sacrificed and plasma and liver tissue were collected. Serum and liver tissue biochemical analyses and histological observation were performed. The human hepatic stellate cell line LX-2 was used to build NASH models by culturing with conditioned medium from WRL-68 liver cells after exposure to MCD medium in vitro. Liver oxidative stress and inflammatory indices and autophagy markers were examined. The results showed that AA suppressed reactive oxygen species (ROS) and inflammation in a NASH mouse model and inhibited the expression of inflammatory cytokines and ROS in LX-2 cells in MCD medium. Furthermore, we found AA stimulated autophagy in mice liver and LX-2, which could be the underlying mechanism of AA in NASH. To further investigate the role of autophagy in LX-2 cells, we found that AA regulated autophagy via the AMPK/mTOR/ULK1 pathway and dorsomorphin, a selective AMPK inhibitor, led to the suppression of AA-induced autophagy. Taken together, our results indicate that AA could be a possible therapy for NASH by inhibiting oxidative stress and stimulating autophagy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18674163	Alisol A 24-acetate		18674-16-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: