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Allantoin Reduces Cell Death Induced by Cisplatin: Possible Implications for Tumor Lysis Syndrome Management

Allantoin Reduces Cell Death Induced by Cisplatin: Possible Implications for Tumor Lysis Syndrome Management

Janaina Fernandes, Gisele Cardoso de Amorim, Tallita Eduarda da Veiga, Jesiel Cardoso, Alberto Cardoso Arruda, Mara Silvia Pinheiro Arruda, Morgana T L Castelo-Branco

J Biol Inorg Chem. 2019 Jun;24(4):547-562.

PMID: 31030325

Abstract:

Massive lysis of tumor mass in cancer patients under chemotherapy regimens generates high levels of uric acid, leading to what is known as tumor lysis syndrome (TLS). Rasburicase, a recombinant urate oxidase, converts urate to allantoin, which is readily excreted by the kidneys. Even though there is a high production of allantoin from urate in cancer patients following rasburicase treatment, there are no studies on how allantoin excess could interfere with chemotherapy. We have evaluated allantoin interference with cisplatin efficiency on the lung cancer cell line H460 in vitro. The cells were treated with cisplatin (33 µM), with or without allantoin, for 48 h, in the presence or absence of UV light, and N-acetyl-L-cysteine (NAC) for 24 h. Cell viability, cell cycle, ROS production, apoptosis and immunoblot assays were performed. We showed that allantoin reduced the apoptosis induced by cisplatin in the H460 cell line. However, the activity of carboplatin and oxaliplatin, betulinic acid, TIBA, UV and H2O2 was not affected by allantoin. NMR spectroscopy showed that allantoin reduces cisplatin activity through direct interaction with cisplatin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP97596	Allantoin		97-59-6	Price

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