Home
Resources
Publications
Allergenicity Reduction of Bovine Milk β-lactoglobulin by Proteolytic Activity of Lactococcus Lactis BMC12C and BMC19H Isolated From Iranian Dairy Products

Allergenicity Reduction of Bovine Milk β-lactoglobulin by Proteolytic Activity of Lactococcus Lactis BMC12C and BMC19H Isolated From Iranian Dairy Products

Rezvan Kazemi, Asghar Taheri-Kafrani, Ahmad Motahari, Reihane Kordesedehi

Int J Biol Macromol. 2018 Jun;112:876-881.

PMID: 29428389

Abstract:

Nowadays health benefits of bioactive food constituents, known as probiotic microorganisms, are a growing awareness. Cow's milk is a nutritious food containing probiotic bacteria. However, milk allergenicity is one of the most common food allergies. The milk protein, β-lactoglobulin (BLG), is in about 80% of all main cases of milk allergies for children and infants. With the aim of screening proteolytic strains of lactic acid bacteria to evaluate their potential for the reduction of allergenicity of the major bovine milk proteins, we isolated new proteolytic strains of cocci lactic acid bacteria from traditional Iranian dairy products. The proteases produced by these strains had strong proteolytic activity against BLG. Proteolysis of BLG, observed after sodium dodecyl sulfate-PAGE, was confirmed by the analysis of the peptide profiles by reversed-phase HPLC. The two isolates were submitted to 16S rDNA sequencing and identified as Lactcoccus lactis subsp. cremoris and Lactcoccus lactis subsp. hordniea. The competitive ELISA experiments confirmed that these isolates, with high proteolytic activity, reduce significantly the allergenicity of BLG. Accordingly, these isolates can reduce the immunoreactivity of bovine milk proteins, which can be helpful for the production of low-allergic dairy products.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9066459	β-Lactoglobulin B from bovine milk		9066-45-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: