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Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In Vivo and In Vitro

Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In Vivo and In Vitro

Giovanni Monastra, Yula Sambuy, Simonetta Ferruzza, Daniela Ferrari, Giulia Ranaldi

Curr Drug Deliv. 2018;15(9):1305-1311.

PMID: 29745333

Abstract:

Background:
Myo-inositol is a natural molecule with important therapeutic applications and an impaired oral absorption may result in a reduced clinical effect. Aim of this study was to determine if the combined oral administration of α-lactalbumin and myo-inositol in healthy subjects, could increase the plasma level of myo-inositol administered alone. In vitro studies on human differentiated intestinal Caco-2 cells were also conducted to identify the mechanisms involved in myo-inositol absorption.
Objective:
The in vivo study was conducted on healthy volunteers in two phases. Subjects received a single oral myo-inositol dose. After 7 days washout, the same subjects were administered a single dose of myo-inositol and α-lactalbumin. Cmax, Tmax and AUC for myo-inositol in plasma were calculated from samples collected at different times. Transepithelial myo-inositol passage, with or without addition of digested α-lactalbumin, was measured in vitro in differentiated Caco-2 cells and compared to transepithelial electrical resistance and phenol red passage.
Results:
The bioavailability of myo-inositol was modified by the concomitant administration of α- lactalbumin. Although peak concentration of myo-inositol at 180 min (Tmax) was similar for both treatments, administration of α-lactalbumin with myo-inositol in a single dose, significantly increased the plasma concentrations of myo-inositol compared to when administered alone. In vitro, myo-inositol absorption in Caco-2 cells was improved in the presence of digested α-lactalbumin, and this change was associated with an increase in tight junction permeability.
Conclusion:
Better myo-inositol absorption when orally administered with α-lactalbumin can be beneficial in non-responder patients. Preliminary in vitro findings suggest that peptides deriving from α- lactalbumin digestion may modulate tight junction permeability allowing increased absorption of myoinositol.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9051290-A	Alpha-Lactalbumin		9051-29-0	Price

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