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Altered Function, Localization and Phosphorylation of Gap Junctions in Rat Liver Epithelial, IAR 20, Cells After Treatment With PCBs or TCDD

Altered Function, Localization and Phosphorylation of Gap Junctions in Rat Liver Epithelial, IAR 20, Cells After Treatment With PCBs or TCDD

Y Bager, M C Lindebro, P Martel, C Chaumontet, L Wärngård

Environ Toxicol Pharmacol. 1997 Sep;3(4):257-66.

PMID: 21781786

Abstract:

Three different PCB-congeners 3,4,5,3',4'-pentachlorobiphenyl (IUPAC no. 126), 2,4,5,2',4',5'-hexachlorobiphenyl (IUPAC no. 153) and 2,4,5,3',4'-pentachlorobiphenyl (IUPAC no. 118) were investigated for possible structure-activity relationships in altering gap junction intercellular proteins. All tested PCB-congeners and TCDD decreased the gap junctional intercellular communication in IAR 20 cells, but at different treatment periods, suggesting different modes of action. The presence of the Cx43-P(2) band, a phosphorylated isoform of Cx43, was associated with a functional communication. A reduced Cx43 mRNA level was noted after 48 h of exposure with PCB 126, PCB 118 and TCDD. In summary, the non dioxin-like PCB 153 can decrease gap junctional intercellular communication rapidly by reducing the phosphorylated isoform of Cx43, whereas the dioxin-like PCB 126 and TCDD reduce the communication slowly by decreasing the mRNA level of Cx43, resulting in a reduced Cx43 protein level (which includes the P(2)-band). The mixed inducing PCB-congener, PCB 118, can act both as the dioxin-like and the non dioxin-like PCBs in gap junction regulation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP35065271-A	PCB No 153		35065-27-1	Price
AP35065271-C	2,2′,4,4′,5,5′-Hexachlorobiphenyl (IUPAC No. 153)		35065-27-1	Price

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