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Ambineela, an Unusual Blue Protein Isolated From the Archaeon Acidianus Ambivalens

Ambineela, an Unusual Blue Protein Isolated From the Archaeon Acidianus Ambivalens

C M Gomes, M Teixeira

Biochem Biophys Res Commun. 1998 Aug 10;249(1):23-5.

PMID: 9705824

Abstract:

A novel blue protein, named ambineela, was isolated from the soluble extract of the thermoacidophilic archaeon Acidianus ambivalens. In solution, the purified protein is a monomer with 50 kDa and has a basic character (pI approximately 8.7). The electronic spectrum shows two bands, centred at 395 and 625 nm (A625/A395 = 0.7). The protein does not contain any transition metal; its blue colour is due to an unidentified non-fluorescent cofactor, covalently bound to it. Ambineela N-terminal sequence exhibits a consensus ADP-binding region, suggesting that its unknown cofactor may comprise this molecule or an analogue.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411120	Fluorescent IEF-Marker pI 8.7 solution			Price

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