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Amperometric Lactate Biosensor for Flow Injection Analysis Based on a Screen-Printed Carbon Electrode Containing Meldola's Blue-Reinecke Salt, Coated With Lactate Dehydrogenase and NAD+

Amperometric Lactate Biosensor for Flow Injection Analysis Based on a Screen-Printed Carbon Electrode Containing Meldola's Blue-Reinecke Salt, Coated With Lactate Dehydrogenase and NAD+

M Piano, S Serban, R Pittson, G A Drago, J P Hart

Talanta. 2010 Jun 30;82(1):34-7.

PMID: 20685431

Abstract:

A biosensor for the measurement of lactate in serum has been developed, which is based on a screen-printed carbon electrode, modified with Meldola's Blue-Reinecke Salt (MBRS-SPCE), coated with the enzyme lactate dehydrogenase NAD(+) dependent (from Porcine heart), and NAD(+). A cellulose acetate layer was deposited on the top of the device to act as a permselective membrane. The biosensor was incorporated into a commercially available, thin-layer, amperometric flow cell operated at a potential of only +0.05 V vs. Ag/AgCl. The mobile phase consisted of 0.2 M phosphate buffer pH 10 containing 0.1 M potassium chloride solution; a flow rate of 0.8 ml min(-1) was used throughout the investigation. The biosensor response was linear over the range 0.55-10 mM lactate; the former represents the detection limit. The precision of the system was determined by carrying out 10 repeat injections of 10 mM l(+)lactic acid standard; the calculated coefficient of variation was 4.28%. It was demonstrated that this biosensor system could be applied to the direct measurement of lactate in serum without pre-treatment; therefore, this would allow high throughput-analysis, at low cost, for this clinically important analyte.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13573165	Reinecke salt		13573-16-5	Price
AP9001609-B	L-Lactic Dehydrogenase from bovine heart		9001-60-9	Price

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