Home
Resources
Publications
Amphiphilic Triblock Copolymer poly(p-dioxanone-co-L-lactide)-block-poly(ethylene Glycol), Enhancement of Gene Expression and Inhibition of Lung Metastasis by Aerosol Delivery

Amphiphilic Triblock Copolymer poly(p-dioxanone-co-L-lactide)-block-poly(ethylene Glycol), Enhancement of Gene Expression and Inhibition of Lung Metastasis by Aerosol Delivery

S R Bhattarai, S Y Kim, K Y Jang, H K Yi, Y H Lee, N Bhattarai, S-Y Nam, D Y Lee, H Y Kim, P H Hwang

Gene Ther. 2007 Mar;14(6):476-83.

PMID: 17122804

Abstract:

We describe the development of an aerosol system for topical gene delivery to the lungs of C57BL/6 mice. This system is based on the combination of the commercial cationic lipid Lipofectin with a novel amphiphilic triblock copolymer, poly(p-dioxanone-co-L-lactide)-block-poly(ethylene glycol) (PPDO/PLLA-b-PEG, and abbreviated in the text as polymeric micelles). After optimizing conditions for DNA delivery to the lungs of mice using the combination of polymeric micelles with Lipofectin and LacZ DNA, we used the Lipofectin/polymeric micelle system to deliver the tumor suppressor gene PTEN to the lungs of C57BL/6 mice bearing the B16-F10 melanoma. Lipofectin/PTEN/polymeric micelles significantly improved gene expression of PTEN in the lungs of mice with no evidence of cell toxicity or acute inflammation. Importantly, lung metastasis, as measured by lung weight, was significantly reduced (P<0.001), as were total tumor foci in the lungs (P<0.001) and size of individual tumor nodules in animals treated with Lipofectin/PTEN/polymeric micelles compared with control animals. Survival time was also extended. These results suggest that the Lipofectin/polymeric micelle system is appropriate for enhancing gene delivery in vivo and that it can be applied as a non-invasive gene therapy for lung cancer.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS74399	Poly(p-dioxanone-co-L-lactide)			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: