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β-Amyrin Synthase (EsBAS) and β-amyrin 28-oxidase (CYP716A244) in Oleanane-Type Triterpene Saponin Biosynthesis in Eleutherococcus Senticosus

β-Amyrin Synthase (EsBAS) and β-amyrin 28-oxidase (CYP716A244) in Oleanane-Type Triterpene Saponin Biosynthesis in Eleutherococcus Senticosus

Hye-Jeong Jo, Jung Yeon Han, Hwan-Su Hwang, Yong Eui Choi

Phytochemistry. 2017 Mar;135:53-63.

PMID: 28012567

Abstract:

Siberian ginseng (Eleutherococcus senticosus) is a woody medical shrub belonging to the Araliaceae family. E. senticosus contains various types of saponins, including oleanane, noroleanane, lupane, and 3,4-secolupane types, depending on the aglycone structure. Oleanane-type triterpenes are the major saponin components in E. senticosus. Two enzymes (β-amyrin synthase and β-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene. In the present study, two full-length cDNAs encoding EsBAS and CYP716A244 were isolated based on transcriptomics analysis of plant leaves. Both β-amyrin synthase (EsBAS) and β-amyrin 28-oxidase (CYP716A244), isolated from E. senticosus, were functionally characterised. β-amyrin production was confirmed by heterologous expression of the EsBAS gene in yeast and tobacco. Oleanolic acid production was confirmed by co-expression of both EsBAS and CYP716A244 in engineered yeast and transgenic tobacco.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP559706	β-Amyrin		559-70-6	Price

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