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An Ammonium-Formate-Driven Trienzymatic Cascade for ω-Transaminase-Catalyzed ( R)-Selective Amination

An Ammonium-Formate-Driven Trienzymatic Cascade for ω-Transaminase-Catalyzed ( R)-Selective Amination

Fei-Fei Chen, Yu-Hui Zhang, Zhi-Jun Zhang, Lei Liu, Jian-Ping Wu, Jian-He Xu, Gao-Wei Zheng

J Org Chem. 2019 Nov 15;84(22):14987-14993.

PMID: 31644289

Abstract:

(R)-Amination mediated by (R)-specific ω-transaminases generally requires costly d-alanine in excess to obtain the desired chiral amines in high yield. Herein, a one-pot, trienzymatic cascade comprising an (R)-specific ω-transaminase, an amine dehydrogenase, and a formate dehydrogenase was developed for the economical and eco-friendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enabled efficient ω-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent ee (>99%); water and CO2 were the only waste products.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP540692-B	Ammonium formate		540-69-2	Price

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