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An Antiserum to the Rat Liver Glucocorticoid Receptor

An Antiserum to the Rat Liver Glucocorticoid Receptor

H J Eisen

Proc Natl Acad Sci U S A. 1980 Jul;77(7):3893-7.

PMID: 7001446

Abstract:

A rabbit immunized with a highly purified preparation of rat liver [3H]triamcinolone-receptor complex developed antibodies to the receptor. Although precipitating reactions were not detected, complexes formed between IgG and the receptor could be detected by Staphylococcus aureus protein A-Sepharose and gel permeation chromatography. IgG was purified and covalently immobilized on Sepharose CL-4B; this affinity matrix adsorbed the ligand-free receptor and both activated and nonactivated forms of the [3H]triamcinolone-receptor complex. Rat liver cytosol proteins adsorbed by control and immune immunoglobulin-Sepharoses were eluted with 0.1 M acetic acid and analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. A protein with molecular weight 78,000 was the major species eluted from immune immunglobulin-Sepharose, and it was not present in eluates from control columns. Rat transcortin, glucocorticoid binder IB, and an estrogen-binding protein from rat liver were not adsorbed by immune IgG-Sepharose. Mouse and hamster liver glucocorticoid receptors showed only limited adsorption. Thus, the antiserum does not crossreact with other major glucocorticoid-binding proteins and demonstrates species specificity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LPLC81205	Protein A, immobilized on Agarose CL-4B from Staphylococcus aureus			Price

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