Home
Resources
Publications
An Enzyme Immunoassay for Prostaglandin E2 Using Biotin-Prostaglandin B2 Conjugate as a Tracer

An Enzyme Immunoassay for Prostaglandin E2 Using Biotin-Prostaglandin B2 Conjugate as a Tracer

S Watanabe, C Yoshida, T Kobayashi, H Okuyama

Biol Pharm Bull. 1997 Jan;20(1):101-3.

PMID: 9013819

Abstract:

Prostaglandin E2 (PGE2) was converted into prostaglandin B2 (PGB2) by alkaline treatment and quantitated by a novel and specific competitive enzyme immunoassay using an anti-PGB2 antibody and a biotin-PGB2 conjugate as a tracer. This assay was relatively specific for PGE1 and PGE2 (n-6 type); the reactivity for PGE3 (n-3 type) was below 2% of that for n-6 type, and was applicable to the quantitation of PGE2 synthesized in lipopolysaccharide-stimulated peritoneal macrophages from mice fed either a high linoleate or a high alpha-linolenate diet.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13367856	Prostaglandin B2		13367-85-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: