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An Immunopotentiator, Ophiopogonin D, Encapsulated in a Nanoemulsion as a Robust Adjuvant to Improve Vaccine Efficacy

An Immunopotentiator, Ophiopogonin D, Encapsulated in a Nanoemulsion as a Robust Adjuvant to Improve Vaccine Efficacy

Ya-Nan Tong, Liu-Yang Yang, Yun Yang, Zhen Song, Liu-Sheng Peng, Ji-Ning Gao, Hao Zeng, Quan-Ming Zou, Hong-Wu Sun, Xu-Hu Mao

Acta Biomater. 2018 Sep 1;77:255-267.

PMID: 30031164

Abstract:

As an ingredient of vaccines, adjuvants are indispensable for enhancing and directly inducing robust and extensive adaptive immune responses associated with vaccine antigens. In this study, we initially determined that a new molecular immunopotentiator, ophiopogonin D (OP-D), enhanced the antibody response to antigen. Because OP-D has certain disadvantages, including poor solubility, we next encapsulated OP-D in a nanoemulsion adjuvant (nanoemulsion-encapsulated OP-D, NOD) using low-energy emulsification methods. The NOD thus produced was small, with an average size of 76.45 nm, and exhibited good distribution (PdI value 0.16), significantly increasing the solubility of OP-D. Furthermore, NOD exhibited reduced cellular toxicity and acute toxicity. Our results showed that a fusion antigen of MRSA (HlaH35LIsdB348-465) formulated with NOD significantly improved humoral and cellular immune responses compared to those observed in the antigen/OP-D and antigen/AlPO4 groups. Compared with antigen/OP-D, the antigen formulated with NOD more effectively promoted antigen uptake by dendritic cells (DCs) and the activation of antigen-presenting cells (APCs). Moreover, the NOD-formulated antigen had ideal protective efficacy in a MRSA sepsis model by inducing more potent antibody responses and a Th1/Th17-biased CD4+ T cell immune response. Therefore, these results suggest that NOD is a promising and robust adjuvant platform for a MRSA vaccine.
Statement of significance:
We first identified a new powerful immunopotentiator, Ophiopogonin D, among dozens of natural products and then used nanotechnology to construct a highly efficient and low toxic adjuvant system (NOD). Our approach intersects natural medicinal chemistry, nanomaterials and immunology, revealing that a strong adjuvant activity of this adjuvant system was verified in vitro and in vivo, and the application of MRSA subunit vaccine model for survival experiments achieved a 100% protection rate. This research illustrate that NOD is a promising and robust adjuvant platform for subunit vaccines.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP945619749	Ophiopogonin D		945619-74-9	Price

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