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An Impedimetric Immunosensor for Highly Sensitive Detection of IL-8 in Human Serum and Saliva Samples: A New Surface Modification Method by 6-phosphonohexanoic Acid for Biosensing Applications

An Impedimetric Immunosensor for Highly Sensitive Detection of IL-8 in Human Serum and Saliva Samples: A New Surface Modification Method by 6-phosphonohexanoic Acid for Biosensing Applications

Elif Burcu Aydın, Mustafa Kemal Sezgintürk

Anal Biochem. 2018 Aug 1;554:44-52.

PMID: 29902421

Abstract:

In this study, we fabricated a sensitive and label-free impedimetric immunosensor based on 6-phosphonohexanoic acid (PHA) modified ITO electrode for detection of interleukin-8 (IL-8) in human serum and saliva. PHA was first employed to cancer biomarker sensing platform. Anti-IL-8 antibody was used as a biorecognition element and the detection principle of this immunosensor was based on monitoring specific interaction between anti-IL-8 antibody and IL-8 antigen. The morphological characterization of each electrode modification step was analyzed by scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) while electrochemical characterization was performed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and single frequency impedance (SFI) techniques. Moreover, the antibody immobilization on the electrode surface was proved Fourier-transform infrared spectroscopy (FTIR) and Raman Spectroscopy. This proposed impedimetric immunosensor exhibited good performances with a wide linear in the range from 0.02 pg/mL to 3 pg/mL as well as a relative low detection limit of 6 fg/mL. The impedimetric immunosensor had a good specificity, stability and reproducibility. This study proved that PHA was a suitable interface material to fabricate an electrochemical biosensor.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP5662759	6-Phosphonohexanoic acid		5662-75-9	Price

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