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An Influenza Hemagglutinin A Peptide Assay Based on the Enzyme-Multiplied Immunoassay Technique

An Influenza Hemagglutinin A Peptide Assay Based on the Enzyme-Multiplied Immunoassay Technique

May L Chiu, Denton Lai, Harold G Monbouquette

J Immunoassay Immunochem. 2011;32(1):1-17.

PMID: 21253966

Abstract:

A practical approach for constructing enzyme-multiplied immunoassay technique (EMIT)-based protein/peptide assays is described. Normally used in small-molecule drug testing, EMIT is a homogeneous assay method that is attractive for its simplicity, sensitivity, and rapidity. The EMIT-based peptide/protein assay was developed by conjugating a cysteine-modified HA peptide (from influenza hemagglutinin A) to the reporter enzyme, glucose-6-phosphate dehydrogenase. The 13-min assay gave a free HA limit of detection of 10 nM and proved effective for detection of a high-molecular-weight model protein tagged with HA. Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP92000765	Influenza Hemagglutinin (HA) Peptide		92000-76-5	Price

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