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An Investigation of Cell Growth and Detachment From Thermoresponsive Physically Crosslinked Networks

An Investigation of Cell Growth and Detachment From Thermoresponsive Physically Crosslinked Networks

Deirdre Healy, Maria Nash, Alexander Gorleov, Kerry Thompson, Peter Dockery, Yury Rochev

Colloids Surf B Biointerfaces. 2017 Nov 1;159:159-165.

PMID: 28783507

Abstract:

The primary aim of this investigation was to determine the biocompatibility and cell culture potential of a newly designed class of thermoresponsive polymers. The attractiveness of these polymers lies in the fact that they swell rather than dissolve when the temperature is reduced below their respective lower critical solution temperature, due to the incorporation of octadecyl methacrylate (ODMA). The ODMA monomer acts as a physical crosslinker, preventing polymer dissolution upon temperature reduction. Two polymers were studied in this investigation poly(N isorpoylacrylamide (NIPAm)(99.25%)-co-ODMA(0.75%)) and poly(NIPAm(65%)-co-N-tert-butylacrylamide (NtBAm)(34.25%)-co-ODMA(0.75%)). Thin thermoresponsive films of the polymers were prepared via spin coating. 3T3 cells were then seeded on the prepared films and cell viability was assessed quantitatively through cell viability and activity assays and qualitatively by light microscopy. Cells were successfully seeded and grown on the poly(NIPAm-co-ODMA) and poly(NIPAm-co-NtBAm-co-ODMA) copolymer films after film modification with cell adhesion promoters (CAPs). Cell sheets successfully detached from the CAP coated poly(NIPAm-co-ODMA) platforms upon temperature reduction.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25639218	Poly(octadecyl methacrylate) solution		25639-21-8	Price

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