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Analysis of Anti-Tumor Antibodies in Mice and Rabbits Induced by Monoclonal Anti-Idiotope Antibodies

Analysis of Anti-Tumor Antibodies in Mice and Rabbits Induced by Monoclonal Anti-Idiotope Antibodies

P Chattopadhyay, J Starkey, S Raychaudhuri

J Immunol. 1991 Sep 15;147(6):2055-62.

PMID: 1890311

Abstract:

Eight different mouse monoclonal anti-idiotope antibodies (mAb2) generated against a mouse monoclonal anti-human melanoma proteoglycan Ag (MPG) antibody (mAb1), MEM136, were tested for their ability to induce anti-MPG responses in mice and rabbits. All Ab2 were idiotypically cross-reactive and combining site-specific as demonstrated by competitive cross-inhibition studies and their ability to inhibit the binding of MEM136 to the melanoma cells, Colo38. However, only two Ab2, IM32 and IM06, were able to induce specific anti-TAA-specific (Ab1') responses in rabbits. When IM32 and IM06 were tested in allogeneic stains of mice for the induction of anti-MPG responses, only IM32 produced an Ab1' response. In mice, the Ab3 response induced by IM32 is idiotypically cross-reactive with its Ab1. Furthermore, the IM32-induced murine Ab3 and MEM136 recognized a similar MPG epitope on the melanoma cells because the Ab3 inhibited the binding of MEM136 to melanoma cells. The Ab3 induced by IM32 and IM06 in rabbits also recognized a similar epitope as the Ab1. In rabbits, the Ab3 response induced by IM32 and IM06 were idiotypically cross-reactive with each other. However, additional studies indicated that the majority of Ab3 induced by IM32 were IM32 Id-specific and lacked IM06 idiotopes. Further experimentation indicated that IM32-induced rabbit Ab3 were biologically active as demonstrated by the ability of the Ab3 to inhibit melanoma cell invasion in a Matrigel assay.

Chemicals Related in the Paper:

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IAR42412738	Anti-TSC2 (ab1) antibody produced in rabbit	Price
IAR42414089	Anti-P4HB (AB1) antibody produced in rabbit	Price
IAR42414431	Anti-MMP19 (AB1) antibody produced in rabbit	Price
IAR4246969	Anti-EDG8 (AB1) antibody produced in rabbit	Price
IAR4248126	Anti-ZDHHC17 (AB1) antibody produced in rabbit	Price
IAR4248961	Anti-MPG antibody produced in rabbit	Price
IAR4249004	Monoclonal Anti-MPG antibody produced in mouse	Price

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