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Analysis of Incurred Crystal Violet in Atlantic Salmon (Salmo Salar L.): Comparison Between the Analysis of Crystal Violet as an Individual Parent and Leucocrystal Violet and as Total Crystal Violet After Oxidation With 2,3-dichloro-5,6-dicyanobenzoquinone

Analysis of Incurred Crystal Violet in Atlantic Salmon (Salmo Salar L.): Comparison Between the Analysis of Crystal Violet as an Individual Parent and Leucocrystal Violet and as Total Crystal Violet After Oxidation With 2,3-dichloro-5,6-dicyanobenzoquinone

D Chan, J A Tarbin, G Stubbings, J Kay, M Sharman

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(1):66-72.

PMID: 22043964

Abstract:

Due to on-going concern about the occurrence of triphenylmethane dye residues in fish destined for human consumption, a depletion study of crystal violet in salmon was carried out. Atlantic salmon less than 12 months old were exposed to crystal violet in fresh water at 15°C and subsequently sampled at 1, 7, 14, 28, 63 and 91 days after exposure. The salmon were then analysed by two analytical methods. In the first method, 2,3-dichloro-5,6-dicyanobenzoquinone (DDQ) was used to oxidise leucocrystal violet to its parent form. Total parent crystal violet was then analysed by LC-MS/MS. In the second method, crystal violet and leucocrystal violet were analysed individually by LC-MS/MS without oxidation. Both methods gave comparable results for total crystal violet concentrations, with a correlation of r(2)=0.69. Statistical treatment for 88 incurred salmon samples showed no significant difference between the two sets of results with t=1.68 and t(crit)=1.99. Up to 98% of crystal violet was metabolised to its leuco form in the salmon after 1 day of exposure and could be detected at significant concentrations (approximately 20 µg kg(-1)) 91 days after exposure. The depletion data also suggest that crystal violet has a half-life of approximately 15-16 days in salmon.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1173023921	Leucocrystal Violet-d6		1173023-92-1	Price

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