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Analysis of Semicarbazide in Baby Food by Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)--in-house Method Validation

Analysis of Semicarbazide in Baby Food by Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)--in-house Method Validation

Scheilla V C de Souza, Roberto G Junqueira, Richard Ginn

J Chromatogr A. 2005 Jun 10;1077(2):151-8.

PMID: 16001551

Abstract:

A method for detection of semicarbazide (SEM) in baby food was validated. SEM was extracted with hydrochloric acid and derivatised with 2-nitrobenzaldehyde, using [15N2,13C] semicarbazide as internal standard. The extract was neutralised, purified on a solid phase extraction cartridge and SEM was determined by reversed phase LC-MS-MS. Linearity was demonstrated in the ranges from 0.1 ng ml(-1) to 1 ng ml(-1) and from 2 ng ml(-1) to 80 ng ml(-1). Matrix effects were non significant for meat-based and significant for apple and rice-based baby foods, in both ranges. Mean recoveries ranged from 87.8% to 107.2% with relative standard deviation from 0.2% to 9.1%, considering both ranges. Limits of detection and quantification were 0.1 microg kg(-1) and 0.25 microg kg(-1), respectively. The results of the validation process demonstrated the method suitability for use in food control.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS211236	2-Nitrobenzaldehyde semicarbazone-13C, 15N2			Price

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