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Analysis of Variations in the Contents of Steroidal Saponins in Fructus Tribuli During Stir-Frying Treatment

Analysis of Variations in the Contents of Steroidal Saponins in Fructus Tribuli During Stir-Frying Treatment

Chao Zhang, Shuyue Wang, Fei Guo, Ting Ma, Longfei Zhang, Linlin Sun, Yingzi Wang, Xuelan Zhang

Biomed Chromatogr. 2020 Apr;34(4):e4794.

PMID: 31944362

Abstract:

Just as natural saponins transform into aglycones, secondary glycosides and their derivatives using biotransformation technology, steroidal saponins may also undergo similar transformation after stir-frying. The purpose of this study was to elucidate the variations and the reasons for these variations in the contents of steroidal saponins in Fructus Tribuli (FT) during a stir-frying treatment. Stir-fried FT was processed in different time-temperature conditions. An UHPLC-MS/MS method was established and fully validated for quantitative analysis. In addition, the simulation processing products of tribuluside A, terrestroside B, terrestrosin K, terrestrosin D and 25R-tribulosin were determined by qualitative analysis using UHPLC-Q-TOF-MS. The established UHPLC-MS/MS method provides a rapid, flexible, and reliable method for the quality assessment of FT. The present study revealed that furostanol saponins with a C22-OH group could transform into corresponding furostanol saponins with a C-20-C-22 double bond (FSDB) via dehydroxylation. Additionally, FSDB could be successively converted into its secondary glycosides via a deglycosylation reaction. The transformation of spirostanol saponins into corresponding aglycones via deglycosylation led to a decrease in spirostanol saponins and an increase in aglycones. The results of this research provided scientific evidence of variation and structural transformation among steroidal saponins. These findings might be helpful for elucidating the processing mechanism of FT.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP79974462	Tribulosin		79974-46-2	Price

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