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Anti-allergic Actions of F-PASA, a Novel Herbal Cocktail, in IgE/antigen-mediated Allergic Responses in RBL-2H3 Cells and Passive Cutaneous Anaphylaxis in Mice

Anti-allergic Actions of F-PASA, a Novel Herbal Cocktail, in IgE/antigen-mediated Allergic Responses in RBL-2H3 Cells and Passive Cutaneous Anaphylaxis in Mice

Jae-Myung Yoo, Kwang Il Park, Ju-Hye Yang, Won-Kyung Cho, Bohyoung Lee, Jin Yeul Ma

Phytomedicine. 2019 Mar 1;55:229-237.

PMID: 30668433

Abstract:

Background:
The anti-inflammatory actions of Polygonum cuspidatum, Angelica gigas, Sophora flavescens and Arctium fruit are well known. Nonetheless, effects of herbal combination (PASA) or its fermentation by microorganisms (F-PASA) on the allergic response remain unknown.
Purpose:
We investigated whether PASA or F-PASA could inhibit IgE/antigen complex (IgE/Ag)-mediated allergic responses.
Methods:
To evaluate and compare anti-allergic actions of PASA and F-PASA, we performed cell viability, β-hexosaminidase activity, ELISA assays for cytokines and eicosanoids, immunoblot analysis, HPLC analysis and passive cutaneous anaphylaxis (PCA) models.
Results:
F-PASA had stronger anti-degranulation actions (IC50, 510.9 µg/ml) than PASA (IC50, 1,261 µg/ml) without cytotoxicity until 2000 µg/ml in IgE/Ag-activated RBL-2H3 cells. Additionally, F-PASA inhibited formation of tumor necrosis factor-α (IC50, 147.4 µg/ml), interleukin-4 (IC50, 213.4 µg/ml), prostaglandin D2 (IC50, 42.40 µg/ml) and leukotriene C4 (IC50, 157.9 µg/ml). Moreover, F-PASA dose-dependently inhibited the phosphorylation and expression of proteins that are related to the FcεRI and arachidonate cascades. Consistent with in vitro studies, F-PASA from 25 to 100 mg/kg also suppressed IgE/Ag-induced PCA reaction more than PASA did in mice. In phytochemical analysis, using PASA and F-PASA, F-PASA showed a higher level of emodin-8-O-β-d-glucoside, whereas the level of arctiin, an artigenin glycoside, was reduced compared with that using PASA.
Conclusion:
These findings indicate that F-PASA, including both artigenin and emodin-8-O-β-d-glucoside, possesses stronger anti-allergic properties. Therefore, F-PASA may be useful as a functional food or as a phytomedicine for allergic diseases.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP186611529-A	IC 261		186611-52-9	Price

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