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Anti-allergic Activity of Emodin on IgE-mediated Activation in RBL-2H3 Cells

Anti-allergic Activity of Emodin on IgE-mediated Activation in RBL-2H3 Cells

Weimin Wang, Qin Zhou, Lu Liu, Keqin Zou

Pharmacol Rep. 2012;64(5):1216-22.

PMID: 23238477

Abstract:

Background:
EMODIN (1,3,8-trihydroxy-6-methylanthraquinone) is a Chinese herbal anthraquinone derivative from the rhizome of rhubarb (Rheum palmatum L.) that exhibits numerous biological activities, such as antitumor, antibacterial, antiinflammatory, and immunosuppressive. In the present studies, the anti-allergic activities of emodin were investigated to elucidate the underlying active mechanisms.
Methods:
The inhibitory effects of emodin on the IgE-mediated allergic response in rat basophilic leukemia (RBL-2H3) cells were evaluated by measuring the release of granules and cytokines. The Ca2+ mobilization in RBL-2H3 cells loaded with the Ca(2+)-reactive fluorescent probe Fluo-4 AM was also measured by laser scanning confocal microscope.
Results:
Emodin inhibited the release of β-hexosaminidase (β-HEX; IC50 = 5.5 μM) and tumor necrosis factor (TNF)-α (IC50 = 11.5 μM) from RBL-2H3 cells induced by 2,4-dinitrophenylated bovine serum albumin (DNP-BSA) and displayed stronger inhibition of β-HEX release than ketotifen fumarate salt (IC50 = 63.8 μM). Emodin at a concentration of 12.5 μM also inhibited the DNP-BSA-induced influx of extracellular Ca2+ in RBL-2H3 cells.
Conclusions:
These results suggested that emodin likely exhibits anti-allergic activities via increasing the stability of the cell membrane and inhibiting extracellular Ca2+ influx.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34580148	Ketotifen fumarate salt		34580-14-8	Price

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