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Anti-erythropoietin Receptor Monoclonal Antibody: Epitope Mapping, Quantification of the Soluble Receptor, and Detection of the Solubilized Transmembrane Receptor and the Receptor-Expressing Cells

Anti-erythropoietin Receptor Monoclonal Antibody: Epitope Mapping, Quantification of the Soluble Receptor, and Detection of the Solubilized Transmembrane Receptor and the Receptor-Expressing Cells

E Morishita, H Narita, M Nishida, N Kawashima, K Yamagishi, S Masuda, M Nagao, H Hatta, R Sasaki

Blood. 1996 Jul 15;88(2):465-71.

PMID: 8695793

Abstract:

A hybridoma cell line producing the monoclonal antibody against erythropoietin receptor (EpoR) was established using the soluble ectodomain of mouse erythropoietin receptor (sEpoR) as an antigen. The monoclonal antibody termed 1G3 bound to the denatured sEpoR. Epitope mapping with peptide library revealed that 1G3 recognized the amino terminal region including the hexapeptide (positions 6 to 11; LeuProAspProLysPhe). The amino acid sequence in this hexapeptide was identical in mice, rats, and humans, and therefore 1G3 bound to EpoR from all of these sources. Using 1G3, we evaluated sEpoR by a sandwich enzyme-linked immunoassay, and EpoR in the solubilized membrane preparation was detected by Western blotting. The cells expressing EpoR were identified with immunochemical staining. We confirmed the presence of EpoR in a neuronal cell line and PC12 cells, and EpoR was expressed in primary cultured hippocampal neurons.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413581	Monoclonal Anti-Erythropoietin antibody produced in rat			Price

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