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[Anti-sense miRNA-21 Oligonucleotide Inhibits Tb 3.1 Human Tongue Squamous Cell Carcinoma Growth in Vitro]

[Anti-sense miRNA-21 Oligonucleotide Inhibits Tb 3.1 Human Tongue Squamous Cell Carcinoma Growth in Vitro]

Ying-jie Tao, Yu Ren, Jia-bin Dong, Lun Zhang, Jun-ping Cheng, Xuan Zhou

Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Feb;46(2):79-83.

PMID: 21426775

Abstract:

Objective:
To investigate the effect of micro RNA-21 (miRNA-21) knocking on the Tb3.1 human tongue squamous cell carcinoma growth.
Methods:
Anti-sense miRNA-21 oligonucleotide was delivered with oligofectamine to suppress Tb 3.1 tongue cancer cell growth in vitro. Real-time polymerase chain reaction (PCR) was conducted to detect the miRNA-21 expression after transfection. Methyl thiazolyl tetrazolium (MTT) assay was used to determine Tb 3.1 cell survival rate. Apoptosis were examined by flow-cytometry. Matrigel matrix and transwell assay were used to determine Tb 3.1 cell colony formation and migration ability. Antigen KI-67 (Ki67), B cell lymphoma (Bcl-2), phosphatase and tensin homolog (PTEN), matrirx metalloproteinase 2 (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) protein expression in Tb 3.1 cell were measured by Western blotting.
Results:
miRNA-21 expression was decreased in miRNA-21 antisense oligonucleotide (ASODN) group. The survival rate of Tb 3.1 cells with AS-miRNA-21 transfection was significantly suppressed (F = 27.02, P = 0.00) and early phase apoptosis (F = 26.641, P = 0.001) induced in Tb 3.1 cell. Ki67, Bcl-2, MMP-2 and MMP-9 protein were down regulated while PTEN and TIMP-1 protein expression was increased.
Conclusions:
Blocking miRNA-21 expression in Tb3.1 cell could suppress cancer cell growth in vitro and miRNA-21 can serve as a novel target candidate for human tongue cancer gene therapy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415796	Protein Phosphatase Inhibitor 1 human			Price

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