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Antibodies Against Nitric Oxide Damaged Poly L-tyrosine and 3-nitrotyrosine Levels in Systemic Lupus Erythematosus

Antibodies Against Nitric Oxide Damaged Poly L-tyrosine and 3-nitrotyrosine Levels in Systemic Lupus Erythematosus

Fozia Khan, Rashid Ali

J Biochem Mol Biol. 2006 Mar 31;39(2):189-96.

PMID: 16584635

Abstract:

Alterations in the amino acid structure or sequence can generate neo-epitopes from self-proteins causing autoaggressive immune attack. Reactive nitrogen species are an important factor that induces post-translational modification of proteins by cellular reduction and oxidation mechanism; cysteinyl-nitrosylation or tyrosine nitration leading to potentially pathogenic pathways. It was thought of interest to investigate the immunogenicity of nitrated poly L-tyrosine vis-á-vis its possible role in the induction of antibodies in systemic lupus erythematosus (SLE). Commercially available poly L-tyrosine was exposed to nitrating species and the damage was monitored by UV spectroscopy and alkaline gel electrophoresis. The results indicated the formation of 3-nitrotyrosine. Nitrated poly L-tyrosine induced higher titre antibodies as compared to the native form. Nitrated poly L-tyrosine was recognized by the autoantibodies present in the sera of patients suffering from SLE by enzyme immunoassays and band shift assay. The possible role of nitrated self-proteins has been discussed in the production of circulating anti-DNA antibodies in SLE.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25619787	Poly-L-tyrosine		25619-78-7	Price

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