Home
Resources
Publications
Antifungal Property of Dihydrodehydrodiconiferyl Alcohol 9'-O-beta-D-glucoside and Its Pore-Forming Action in Plasma Membrane of Candida Albicans

Antifungal Property of Dihydrodehydrodiconiferyl Alcohol 9'-O-beta-D-glucoside and Its Pore-Forming Action in Plasma Membrane of Candida Albicans

Hyemin Choi, Jaeyong Cho, Qinglong Jin, Eun-Rhan Woo, Dong Gun Lee

Biochim Biophys Acta. 2012 Jul;1818(7):1648-55.

PMID: 22406553

Abstract:

The aims of this study wereto investigate the antifungal activity as a bioactive property of dihydrodehydro-diconiferyl alcohol 9'-O-3-D-glucoside (DDDC9G) and the mode of action(s) involved in its effect. Antifungal susceptibility testing showed that DDDC9G possessed potent antifungal activities toward various fungal strains with almost no hemolytic effect. To understand the antifungal mechanism(s) of DDDC9G, we conducted the following experiments in this study using Candida albicans. Fluorescence experiments using the probe 1,6-eiphenyl-1, 3, 5-hexatriene (DPH) and propidium iodide suggested that DDDC9G perturbed the fungal plasma membrane. Consecutively, the analysis of the transmembrane electrical potential (DeltaPsi) with 3, 3'-dipropylthiadicarbocyanine iodide [DiSC3(5)] and bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)] indicated that DDDC9G induced membrane-depolarization. Furthermore, model membrane studies were performed wiith rhodamine-labeled giant unilamellar vesicles (GUVs), calcein encapsulating large unilamellar vesicles (ILUVs), and FITC-dextran (FD) loaded LUVs. These results demonstrated that the antifungal effects of DDDC9G upon the fungal plasma membrane were through the formation of pores with the radii between 0.74 nm and 1.4 nm. Finally, in three dimensional (3D) flow cytometric contour plots, a reduced cell size was observed as a result of osmolarity changes from DDDC9G-induced structural and functional membrane damages.Therefore, the present study suggests that DDDC9G exerts its antifungal effect by damaging the membrane through pore formation in the fungal plasma membrane.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP53213948	3,3′-Dipropylthiadicarbocyanine iodide		53213-94-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: