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Antigens of Monoclonal Antibody NB3C4 Are Novel Markers for Oligodendrocytes

Antigens of Monoclonal Antibody NB3C4 Are Novel Markers for Oligodendrocytes

K Yoshimura, F Kametani, Y Shimoda, K Fujimaki, Y Sakurai, K Kitamura, H Asou, M Nomura

Neuroreport. 2001 Feb 12;12(2):417-21.

PMID: 11209960

Abstract:

We produced NB3C4, a novel monoclonal antibody specific for oligodendrocytes, using human neuroblastoma IMR-32 cells. NB3C4 specifically recognized oligodendrocytes in the CNS, although it bound to neuroblastoma IMR-32 cells and oligodendrocytes in vitro. Double immunofluorescence staining of rat brain using NB3C4 and anti-GST-pi, anti-glial fibrillary acidic protein (GFAP), or anti-neurofilament 200 (NF) antibody revealed that anti-GST-pi antibody identified an oligodendrocyte marker recognizing NB3C4-positive cells, while both anti-GFAP and anti-NF antibody did not. Western blotting of rat brain homogenates showed that NB3C4 bound three proteins of 22-28 kDa, while the anti-GST-pi recognized a 27 kDa protein. Therefore, antigens recognized by NB3C4 could be novel markers for oligodendrocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4247688	Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse			Price
IAR4247742	Monoclonal Anti-Neurofilament 200 antibody produced in mouse			Price

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