Home
Resources
Publications
Antimicrobial Activity of Phyllanthus Amarus Schumach. & Thonn and Inhibition of the NorA Efflux Pump of Staphylococcus Aureus by Phyllanthin

Antimicrobial Activity of Phyllanthus Amarus Schumach. & Thonn and Inhibition of the NorA Efflux Pump of Staphylococcus Aureus by Phyllanthin

Alessandra Maria Braga Ribeiro, Jonas Nascimento de Sousa, Luciana Muratori Costa, Felipe Araújo de Alcântara Oliveira, Raimunda Cardoso Dos Santos, Aline Suelen Silva Nunes, Wanderson Oliveira da Silva, etc.

Microb Pathog. 2019 May;130:242-246.

PMID: 30876871

Abstract:

The aim of this study was to evaluate the antimicrobial activity of ethanoic extract of P. amarus (PAEE) and its compound Phyllanthin, as well as, investigate if these natural products could modulate the fluoroquinolone-resistance in S. aureus SA1199-B by way of overexpression of the NorA efflux pump. Microdilution tests were carried out to determine the minimal inhibitory concentration (MIC) of the PAEE or Phyllanthin against several bacterial and yeast strains. To evaluate if PAEE or Phyllanthin were able to act as modulators of the fluoroquinolone-resistance, MICs for Norfloxacin and ethidium bromide were determined in the presence or absence of PAEE or Phyllanthin against S. aureus SA1199-B. PAEE showed antimicrobial activity against Gram-negative strains, meanwhile Phyllanthin was inactive against all strains tested. Addition of PAEE or Phyllanthin, to the growth media at sub-inhibitory concentrations enhanced the activity of the Norfloxacin as well as, Ethidium Bromide, against S. aureus SA1199-B. These results indicate that Phyllanthin is able to modulate the fluoroquinolone-resistance possibly by inhibition of NorA. This hypothesis was supported by in silico docking analysis which confirmed that Phyllantin is a NorA ligand. Thus, this compound could be used as a potentiating agent of the Norfloxacin activity in the treatment of infections caused by fluoroquinolone-resistant S. aureus.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP10351889	Phyllanthin		10351-88-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: