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Antimicrobial Photodynamic Inactivation Is Potentiated by the Addition of Selenocyanate: Possible Involvement of Selenocyanogen?

Antimicrobial Photodynamic Inactivation Is Potentiated by the Addition of Selenocyanate: Possible Involvement of Selenocyanogen?

Liyi Huang, Weijun Xuan, Andrzej Zadlo, Anna Kozinska, Tadeusz Sarna, Michael R Hamblin

J Biophotonics. 2018 Aug;11(8):e201800029.

PMID: 29488327

Abstract:

We previously showed that antimicrobial photodynamic inactivation (aPDI) of Gram-positive and Gram-negative bacteria mediated by the phenothiazinium dye, methylene blue (MB), was potentiated by the addition of potassium thiocyanate (10 mM). The mechanism was suggested to involve a singlet oxygen-mediated reaction with SCN to form sulfite and cyanide and then to produce sulfur trioxide radical anion. We now report that potassium selenocyanate (concentrations up to 100 mM) can also potentiate (up to 6 logs of killing) aPDI mediated by a number of different photosensitizers (PS): MB, rose bengal and 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin dihydrochloride (as low as 200 nM). When a mixture of selenocyanate with these PS in solution was illuminated and then bacteria were added after the light, there was up to 6 logs of killing (Gram-negative > Gram-positive) but the antibacterial species decayed rapidly (by 20 minutes). Our hypothesis to explain this antibacterial activity is the formation of selenocyanogen (SeCN)2 by reaction with singlet oxygen (1 O2 ) as shown by quenching of 1 O2 by SeCN and increased photoconsumption of oxygen. The fact that lead tetraacetate reacted with SeCN (literature preparation of (SeCN)2 ) also produced a short-lived antibacterial species supports this hypothesis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3425465	Potassium selenocyanate		3425-46-5	Price

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