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Antiparasitic Efficacy of Gracillin and Zingibernsis Newsaponin From Costus Speciosus (Koen Ex. Retz) Sm. Against Ichthyophthirius Multifiliis

Wei Zheng, Chun-Mei Yan, Ya-Bin Zhang, Ze-Hong Li, Zhongqiang Li, Xiu-Ying Li, Zhang-Wei Wang, Xiulan Wang, Wei-Qiang Chen, Xin-Hai Yu

Parasitology. 2015 Mar;142(3):473-9.

PMID: 25140457

Abstract:

The present study aims to evaluate the antiparasitic activity of active components from Costus speciosus against Ichthyophthirius multifiliis. Bioassay-guided fractionation was employed to identify active compounds from C. speciosus yielding 2 bioactive compounds: Gracillin and Zingibernsis newsaponin. In-vitro assays revealed that Gracillin and Zingibernsis newsaponin could be 100% effective against I. multifiliis at concentrations of 0.8 and 4.5 mg L(-1), with median effective concentration (EC50) values of 0.53 and 3.2 mg L(-1), respectively. All protomonts and encysted tomonts were killed when the concentrations of Gracillin and Zingibernsis newsaponin were 1.0 and 5.0 mg L(-1). In-vivo experiments demonstrated that fish treated with Gracillin and Zingibernsis newsaponin at concentrations of 1.0 and 5.0 mg L(-1) carried significantly fewer parasites than the control (P<0.05). Mortality of fish did not occur in the treatment group (Zingibernsis newsaponin at 5.0 mg L(-1)) during the trial, although 100% of untreated fish died. Acute toxicities (LD50) of Gracillin and Zingibernsis newsaponin for grass carp were 1.64 and 20.7 mg L(-1), respectively. These results provided evidence that the 2 compounds can be selected as lead compounds for the development of new drugs against I. multifiliis.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP19083002 Gracillin Gracillin 19083-00-2 Price
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