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Application of a Quantitative

Application of a Quantitative

Rie Tanaka, Risa Inagaki, Naoki Sugimoto, Hiroshi Akiyama, Akito Nagatsu

J Nat Med. 2017 Jan;71(1):315-320.

PMID: 27631429

Abstract:

A quantitative 1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d 4 to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by 1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27741011	Geniposidic acid		27741-01-1	Price

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