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Application of Diffusion-Edited and Solvent Suppression ¹H-NMR to the Direct Analysis of Markers in Valerian-Hop Liquid Herbal Products

Application of Diffusion-Edited and Solvent Suppression ¹H-NMR to the Direct Analysis of Markers in Valerian-Hop Liquid Herbal Products

Jose M Prieto, Maria Mellinas-Gomez, Mire Zloh

Phytochem Anal. Mar-Apr 2016;27(2):100-6.

PMID: 26763752

Abstract:

Introduction:
The rising trend to consume herbal products for the treatment and/or prevention of minor ailments together with their chemical and pharmacological complexity means there is an urgent need to develop new approaches to their quality and stability.
Objectives:
This work looks at the application of one-dimensional diffusion-edited (1)H-NMR spectroscopy (1D DOSY) and (1)H-NMR with suppression of the ethanol and water signals to the characterisation of quality and stability markers in multi-component herbal medicines/food supplements.
Material and methods:
The experiments were performed with commercial tinctures of Valeriana officinalis L. (valerian), expired and non-expired, as well as its combination with Hummulus lupulus L. (hops), which is one of the most popular blends of relaxant herbs. These techniques did not require purification or evaporation of components for the qualitative analysis of the mixture, but only the addition of D2 O and TSP.
Results:
The best diagnostic signals were found at δ 7 ppm (H-11, valerenic acid), δ 4.2 ppm (H-1, hydroxyvalerenic acid) and δ 1.5-1.8 ppm (methyl groups in prenylated moieties, α-acids/prenylated flavones).
Conclusion:
This work concludes on the potential value of 1D DOSY (1)H-NMR to provide additional assurance of quality in complex natural mixtures.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1619165	Hydroxyvalerenic acid		1619-16-5	Price

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