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Application of β-glucuronidase-immobilised Silica Gel Formulation to Microfluidic Platform for Biotransformation of β-glucuronides

Application of β-glucuronidase-immobilised Silica Gel Formulation to Microfluidic Platform for Biotransformation of β-glucuronides

Cahit Muderrisoglu, Sayit Sargin, Ozlem Yesil-Celiktas

Biotechnol Lett. 2018 May;40(5):773-780.

PMID: 29497885

Abstract:

Objective:
To improve the efficiency of reactions of β-glucuronidase (GUS)-assisted glucuronic acid (GluA) removal within a microfluidic system.
Results:
β-glucuronidase from Helix pomatia was immobilised and characterised in silica-based sol-gel monoliths. Efficiency of the GUS-doped silica monoliths was tested for hydrolysis of p-Nitrophenyl-β-D-glucuronide (pNP-GluA) in both ml-scaled medium via batch reactions and microfluidic environment via continuous-flow reactions. In the microfluidic platform, within a duration of 150 min of continuous operation (flow rate: 1 µL/min), the obtained highest pNP yield was almost 50% higher than that of the corresponding batchwise reaction. However, increased flow rates (3, 5, and 10 µL/min) resulted in lower conversion yields compared to 1 µL/min. The microfluidic platform demonstrated continuous hydrolytic activity for 7 days with considerable reaction yields while using a small amount of the enzyme.
Conclusion:
These results revealed that usage of the microreactors has considerable potential to efficiently obtain bioactive GluA-free aglycons from various plant-derived β-glucuronides for pharmaceutical applications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001450-B	β-Glucuronidase from Helix pomatia		9001-45-0	Price

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