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Application of Lipase Immobilized on the Biocompatible Ternary Blend Polymer Matrix for Synthesis of Citronellyl Acetate in Non-Aqueous Media: Kinetic Modelling Study

Application of Lipase Immobilized on the Biocompatible Ternary Blend Polymer Matrix for Synthesis of Citronellyl Acetate in Non-Aqueous Media: Kinetic Modelling Study

Kirtikumar C Badgujar, Bhalchandra M Bhanage

Enzyme Microb Technol. 2014 Apr 10;57:16-25.

PMID: 24629263

Abstract:

This work reports the use of new support for immobilization of lipase Burkholderia cepacia (BCL) matrix made up of polylactic acid (PLA), chitosan (CH), and polyvinyl alcohol (PVA). Initially lipase from various microbial sources and immobilization support composition was screened to obtain a robust biocatalyst. Among various biocatalysts preparation, the PLA:PVA:CH:BCL (1:6:1:2) was worked as a robust biocatalyst for the citronellyl acetate synthesis. Various reaction parameters were studied in detail to obtain the suitable reaction conditions for model citronellyl acetate synthesis reaction. Various kinetic parameters such as r(max), K(i(citronellol)), K(m(citronellol)), K(m(vinyl acetate)) were determined using non-linear regression analysis for the ternary complex as well as bi-bi ping-pong mechanism. The experimental results and kinetic study showed that citronellyl acetate synthesis catalyzed by immobilized lipase BCL followed the ternary complex mechanism with inhibition by alcohol (citronellol). The energy of activation for citronellyl acetate synthesis was found to be lower for immobilized lipase (8.9 kcal/mol) than aggregated lipase (14.8 kcal/mol) enzyme. The developed biocatalyst showed four to fivefold higher catalytic activity and excellent recyclability (up to six cycles) than the aggregated lipase.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP150845	Citronellyl acetate		150-84-5	Price

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